ProTest

ProTest is a self-contained Biological Indicator for use in either Steam or Ethylene Oxide (EO) sterilization. Each unit is made up of a plastic container with cap, a crushable glass ampoule with recovery media, and a paper strip inoculated with spores. ProTest is available for healthcare and industrial use.

ProTest EO (Green) 48-Hour Incubation Suitable for use in a hospital or industrial Ethylene Oxide sterilization. Contains B. atrophaeus1 spores Log 6   Catalog # PT-1-6-25 (25 BIs)   Catalog # PT-1-6-100 (100 BIs) Intended for use in EO 600mg/L, 60%RH, 54°C Sterilization failure evident by a dramatic green to yellow color change and/or turbidity.

ProTest Steam (Purple) 24-Hour Incubation Intended for use in steam 121°C gravity and 134° C flash gravity (3 and 10 minute) cycles and 121-135°C pre-vacuum cycles. Suitable for use in hospital or industrial Steam Sterilization. Contains G. stearothermophilus1 spores Log 5   Catalog # PT-3-5-25 (25 BIs)   Catalog # PT-3-5-100 (100 BIs) Log 6   Catalog # PT-3-6-25 (25 BIs)   Catalog # PT-3-6-100 (100 BIs) Sterilization failure evident by a dramatic purple to yellow color change and/or turbidity.

Activation

 NOTE: The crusher works the same whether you are using ProTest (pictured) or ProSpore2

Control

The control unit should exhibit turbidity and/or a color change to or toward yellow. If the control unit does not show signs of growth, consider the test invalid.

Test

A failed sterilization cycle is indicated by turbidity and/or a color change to or toward yellow. A test unit that retains its original color (green for ProTest EO and purple for ProTest Steam) indicates that sterilization parameters have been met.

Certification

Each lot of ProTest is certified for population, species, D-value, z-value (where applicable), purity and expiry. Shelf life is 18 months from the date of manufacture. The above catalog numbers for ProTest comply with the AAMI ST19/EN 866-7 (Steam) and AAMI ST24/EN 866-8 (Ethylene Oxide).

Related Information

Note:
¹ Derived from recognized reference strains as specified within USP, ISO or EN guidelines. These products are not certified as being representative of the reference strain and they should therefore not be used where a reference culture is specified.